Shathra hailed from Earth-001 before it became Loomworld. Daughter of Goddesses Oshtur and Gaea, she was tasked by them with designing the Celestial Map of Humanity. After arduous labors, Shathra created the "Great Nest," to which her younger sister Neith saw a problem in the design and created a "Great Web" of her own. An inspecting Oshtur and Gaea were impressed with Neith's design and offered to help her bring it to fruition by making her the shaper of the Multiverse. Neith agreed on the condition that Shathra would not mind and Shathra wished her well, even though her own heart had become blackened with jealousy and rage. Once Neith constructed the Web of Life and Destiny presented to other gods, Shathra became incensed by the "chaos" of free will and disgusted with the Spiders that inhabited it. Her rage and envy manifesting fully, Shathra turned into a wasp-like dark goddess, determined to one day have her spider-wasps take revenge on all Spiders and make her Great Nest a reality to supplant the Great Web.
Shathra learned of Spider-Man when he strayed from the safe path during a visit to the Astral Plane to locate the inter-dimensional kidnapper known as Shade. Shathra immediately realized Spider-Man's true nature and determined that he would make the perfect meal for her hungry young hatchlings. Following Spider-Man's scent back to Earth, Shathra quickly engaged her chosen victim in a battle that demonstrated her clearly superior speed and strength. Though Spider-Man was able to use his superior knowledge of New York to find a temporary escape, he knew that Shathra would soon locate him once more.
Shathra turned the game in a new direction, changing to a human form and adopting the name “Sharon Keller”. Posing as Spider-Man's jilted lover, she went on live TV to reveal a series of convincing and humiliating details. Spurred by Mary Jane Watson's distress, Peter Parker rose to the taunt, gatecrashes the studio interview, and attacked Sharon Keller in full view. Fortunately, Shathra reverted to her Spider-Wasp form, but her gambit had achieved its goal of pushing Spider-Man to act on pure instinct, bringing the conflict to the simple level of “natural order”, in which the Spider-Wasp is the hunter and the Spider is the meal.
Spider-Man managed barely to avoid descending into mindless fury and though drugged by Shathra's paralyzing agent, managed to escape to brief safety. He was rescued by Ezekiel Sims, who took Spider-Man to a sacred temple in Ghana and helped Peter to set a trap for Shathra. Spider-Man now consciously allowed his arachnid side to take the forefront, or enough so to capture the Spider-Wasp and feed her to whatever spider dwelt in the depths of the temple. The food chain, it seems, can run in both directions.
Years later, Madame Web sent a letter to Kaine Parker in Houston telling him to get out of town secretly encoding a message that Shathra was coming, but the letter never got to him so he was ignorant of the incoming threat. She then appeared to Kaine in the form of his girlfriend Annabelle Adams and after sleeping with him revealed her true form to him. Kaine had to succumb to his form of The Other to kill Shathra, who had revealed she wanted Kaine to use him to feed her "children."
While repairing the Great Web on the Loomworld, Spiderling inadvertently touched the Primal Strand of the Web, releasing Shathra. Recognizing her as the broodling of Peter Parker, Shathra sent Spider-Wasps to sting her, corrupting Annie and transforming her into Shathra's Nestling. Shathra then personally turned the Noir Spider-Man while sending swarms to turn other spiders such as Gwen and Mayday. With preparations ready, Shathra bade Nestling to open a portal to send a swarm to Earth-616 and sent Noir, who killed Spider-Woman before bringing the 616 Spiders, Miles, and Silk, as well as Morlun to her Great Nest.
